Endocrinology (USA)

Endocrinology EIA tests for diabetes and other hormones.

All the following tests are available in the USA.

Diabetes
Intact Proinsulin EIA: is a two-site enzyme-immunoassay (EIA), employing a specific solid phase antibody immobilised on microtitre wells and a soluble antibody labelled with biotin. With results in 4 hours, the assay is easy to use and features high specificity (no cross reactivity with split proinsulins, insulin or c-peptide) and sensitivity, with a limit of detection of 0.3 pmol/L. The Lab21 assay utilises monoclonal antibodies to minimise the cross reactivity with split proinsulins that other assay have historically suffered with.

Intact Proinsulin CLIA: a chemiluminecent test for the quantification of the major precursor molecules (intact proinsulin, 31-32, 32-33, 64-65 and 65-66 split proinsulins) produced during insulin biosynthesis in the blood. In patients with type 2 diabetes, relatively greater proportions of intact proinsulin, 32-33 split proinsulin and des 31-32 split proinsulin find their way into peripheral blood. Only small amount of unmodified proinsulin finds its way into the circulation in normal subjects.

Intact Proinsulin EIA: a two-site enzyme-immunoassay (EIA), employing a specific solid phase antibody immobilised on microtitre wells and a soluble antibody labelled with biotin. With results in 4 hours, the assay is easy to use and features high specificity (no cross reactivity with split proinsulins, insulin or c-peptide) and sensitivity, with a limit of detection of 0.3 pmol/L. The Lab21 assay utilises monoclonal antibodies to minimise the cross reactivity with split proinsulins that other assay have historically suffered with.

C-Peptide CLIA: a two-site immunoassay, employing a C-peptide-specific solid phase antibody immobilised on microtitre wells, and a soluble antibody labelled with a chemiluminescent acridinium ester. With results in under 3 hours, the assay is easy to use and features high specificity (negligible cross reactivity with insulin (0%) or c-peptide (2%)), a high sensitivity (5 pmol/L) and a wide working range, 0-6000 pmol/L, reducing the need for regular sample dilution.

Insulin CLIA: a two-site immunoassay, employing an insulin-specific solid phase antibody immobilised on microtitre wells, and a soluble antibody labelled with a chemiluminescent acridinium ester. With results in under 3 hours, the assay is easy to use and features high specificity (negligible cross reactivity with intact proinsulin, 32-33 split proinsulin and des 31-32 split proinsulin) making it ideal for determining HOMA scores. Therapeutic insulin analogues can be measured using this assay.

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Other hormone EIA Tests:

DHEA-S: Indication: Adrenal hyperplasia, differential diagnosis of hirsutism. Quantitative determination of DHEA-S (Dehydroepiandrosterone sulphate) in human serum or plasma. Reaction: 60/15 min, 450nm, 37°C/RT.

Progesterone: Indication: Hypothalamic pituitary insufficiency, gonadal insufficiency. Quantitative determination of Progesterone in human serum or plasma.
Reaction: 60/15 min, 450nm, 37°C/RT.

17-Beta Estradiol: Indication: Evaluation of ovarian functions. Quantitative determination of 17-ß Estradiol in human serum or plasma. Reaction: 120/30 min, 450nm, 37°C/RT.

hGH: Indication: Hypothalamic pituitary retarded excessive growth, hypothalamic pituitary dwarfism or gigantism quantitative determination of hGH (human growth hormone) in human serum.
Reaction: 60/15 min, 450 nm, RT.

Prolactin: Indication: Disease of the human breast, corpus luteum insufficiency, female sterility, hypothalamic pituitary diseases. Quantitative determination of Prolactin in human serum or plasma.
Reaction: 30/30/15 min, 450-620nm, RT.

FSH: Indication: Gonadal insufficiency, hypothalamic pituitary insufficiency. Quantitative determination of FSH (follicle stimulating hormone) in human serum or plasma. Reaction: 60/10 min, 450nm, RT.

LH: Indication: Gonadal insufficiency, hypothalamic pituitary insufficiency. Quantitative determination of LH (Luteinising hormone, lutropin) in human serum or plasma. Reaction: 60/15 min, 450nm, RT.

Products available:

Description	Quantity
Intact Proinsulin EIA	96 Tests	CE
Intact Proinsuliun CLIA	96 Tests	CE
Total Proinsulin CLIA	96 Tests	CE
C-Peptide CLIA	96 Tests	CE
Insulin CLIA	96 Tests	CE
DHEA-S	96 Tests
Progesterone	96 Tests
17-Beta Estradiol	96 Tests
hGH	96 Tests
Prolactin	96 Tests
FSH	96 Tests
LH	96 Tests

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